pbpE [2018-07-17 19:07:34]

penicillin-binding protein PBP 4* (spore cortex)

locus: BSU34440

pI: 4.84

mw: 51.27 kDa

protein length: 451 aa

gene length: 1353 bp

function: endopeptidase

product: penicillin-binding protein PBP 4* (spore cortex)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,534,118 → 3,535,473

The protein

Protein family

• beta-lactamase family (according to Swiss-Prot)

Structure

• [PDB|3TG9] (from ''Bacillus halodurans'', 27% identity, 59% similarity)

[SW|Localization]

• spore cortex

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]-[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]
  description: [Pubmed|8491712]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|8491712,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulation

  • induced by cell wall stress ([protein|search|SigW]) [Pubmed|8491712,12207695]

Biological materials

Mutant

• BKE34440 (Δ[gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA
• BKK34440 (Δ[gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA

GFP fusion

• 3106 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]::pSG5046 (cat P_xyl-gfpa-[gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]1-761) [pubmed|15758244], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

• 8491712,12533473,9987136,19063962,12884008,12207695,7592498,20817675,15378759,15758244