pbpE

penicillin-binding protein PBP 4*

Locus: BSU_34440

Isoelectric point: 4.84

Molecular weight: 51.27 kDa

Protein length: 451 aa

Gene length: 1356 bp

Function: endopeptidase

Product: penicillin-binding protein PBP 4*

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,534,118 → 3,535,473

The protein

Protein family

• beta-lactamase family (according to UniProt)

Structure

• 3TG9 (from Bacillus halodurans, 27% identity, 59% similarity)

Localization

• cell membrane (according to UniProt)
• distinct foci and bands at cell periphery PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pbpE-racX
  Description: PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in SigW regulon

  Regulation

  • induced by cell wall stress (SigW) PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE34440 (ΔpbpE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA
• BKK34440 (ΔpbpE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA

GFP fusion

• 3106 trpC2 pbpE::pSG5046 (cat P_xyl-gfpa-pbpE1-761) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

References

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