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motB [2017-10-25 11:53:56]
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motB [2017-10-25 11:53:56]

H+-coupled MotA-MotB flagellar stator
locus
BSU13680
pI
6.65
mw
29.33 kDa
protein length
261 aa Sequence Blast
gene length
783 bp Sequence Blast
function
motility and chemotaxis
product
motility protein (flagellar motor rotation)
essential
no
synonyms
mot
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,433,676 → 1,434,461

    Phenotypes of a mutant

  • loss of swimming and swarming motility [Pubmed|24296669]
  • mucoid phenotype due to the [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P activated overexpression of the ''[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]'' operon and resulting overproduction of poly-gamma-glutamate [Pubmed|24296669,23888912]

    • The protein

    Protein family

  • OmpA-like domain (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|6617D785D08C5919F0B774D2AF9EA6A84215486B|MotS]

    • Modification

  • protonation of Asp-24 is essential for torque generation [Pubmed|23888912,9573160]

    • Expression and Regulation

    Operons

    genes
    [gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]-[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]
    description
    [Pubmed|6313226]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|6313226], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
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    Biological materials

    Mutant

  • available in [[Nicola Stanley-Wall]]'s lab [Pubmed|23888912]
  • BKE13680 (Δ[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT, downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG
  • BKK13680 (Δ[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT, downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG

    • References

  • 6313226,24296669,23888912,9573160,25273986,28378843