gsiB

general stress protein, prevents enzyme inactivation upon freeze-thaw treatments

locus: BSU_04400

pI: 5.14

mw: 13.66 kDa

protein length: 123 aa

gene length: 372 bp

function: response to water deficits

product: general stress protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 494,506 → 494,877

The protein

Structure

• [PDB|3JQH] (human glycan binding receptor, 31% identity) [pubmed|19835887]

Expression and Regulation

Operons

• genes: [gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]
  description: [Pubmed|1378051]

  sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [Pubmed|11157964], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• 1A735 ( ''gsiB''::''kan''), [Pubmed|1378051], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A735&Search=1A735 BGSC]
• BKE04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA, downstream forward: _UP4_TAATCAAAAGCATAAAGGCA
• BKK04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA, downstream forward: _UP4_TAATCAAAAGCATAAAGGCA

References

• 9669336,12884008,7651322,11544224,1378051,21421783,23861025,11918677,19835887,29914988