yknY [2019-09-16 09:37:49]

[SW|ABC transporter ](ATP-binding protein, exporter) for the export of the [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

locus: BSU_14360

pI: 5.82

mw: 25.12 kDa

protein length: 230 aa

gene length: 693 bp

function: resistance to [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

product: [SW|ABC transporter ](ATP-binding protein, exporter)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,505,416 → 1,506,108

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|YtrE], [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE], [protein|8B4267532B9788AEB5EF39E2695C1FDA494C7055|YxdL], [protein|464DD1855E6B3195EE242637612A16C73FF32FC0|PsdA], [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA], [protein|0F8048267EC038D8CF673317D969BA27735473A7|YvrO]

Structure

• [PDB|1L2T] (from Methanocaldococcus jannaschii, 52% identity) [pubmed|12150914]

[SW|Localization]

• membrane associated (via [protein|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|YknZ]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU]-[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]-[gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|22383849]

• genes: [gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|9987136]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|12076816], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|12076816]

Biological materials

Mutant

• MGNA-B350 (yknY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1349 NBRP B. subtilis, Japan]
• BKE14360 (Δ[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGCACATTAGAAAGCTGAA, downstream forward: _UP4_TCCGGACAAAGGAGTGTGGG
• BKK14360 (Δ[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGCACATTAGAAAGCTGAA, downstream forward: _UP4_TCCGGACAAAGGAGTGTGGG

References

• 10092453,16629676,9987136,12076816,12150914