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ccpC [2017-12-15 16:53:38]
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ccpC [2017-12-15 16:53:38]

transcriptional repressor of citZ and citB
Locus
BSU14140
Isoelectric point
5.93
Molecular weight
33.76 kDa
Protein length
293 aa Sequence Blast
Gene length
879 bp Sequence Blast
Function
regulation of tricarboxylic acid branch of the TCA cycle
Product
transcriptional repressor (LysR family)
Essential
no
Synonyms
ykuM

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,486,045 → 1,486,926

The protein

Expression and Regulation

Operons

Genes
Description

Sigma factors

  • SigA: sigma factor, in SigA regulon
  • Regulatory mechanism

  • CcpC: repression, (molecular inducer: citrate) PubMed, in CcpC regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed in the presence of glucose and glutamate (CcpC) PubMed
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    Description

    Regulatory mechanism

  • Spo0A: activation, by Spo0A~P PubMed, in Spo0A regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
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    Biological materials

    Mutant

  • MGNA-A774 (ykuM::erm), available at the NBRP B. subtilis, Japan
  • GP206 (aphA3) & GP1442 (aphA3) , available in Stülke lab
  • BKE14140 (ΔccpC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
  • BKK14140 (ΔccpC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
  • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Stülke lab
  • LacZ fusion

  • pGP707 (in pAC5), available in Stülke lab, pGP711 (in pAC6), available in Stülke lab
  • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • References

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