ccpC [2017-12-15 16:53:38]

transcriptional repressor of citZ and citB

locus: BSU14140

pI: 5.93

mw: 33.76 kDa

protein length: 293 aa

gene length: 879 bp

function: regulation of tricarboxylic acid branch of the TCA cycle

product: transcriptional repressor (LysR family)

essential: no

synonyms: ykuM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

Gene

Coordinates: 1,486,045 → 1,486,926

The protein

Protein family

• [SW|LysR family]

Expression and Regulation

Operons

• genes: [gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
  description: [Pubmed|16395550]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FE15A41A58A8177280817CA3825764C39185021A|CcpC]: repression, (molecular inducer: citrate) [Pubmed|11985717], in [regulon|FE15A41A58A8177280817CA3825764C39185021A|CcpC regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed in the presence of glucose and glutamate ([protein|search|CcpC]) [Pubmed|11985717]

• genes: [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
  description: [Pubmed|16395550]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-A774 (ykuM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/774 NBRP B. subtilis, Japan]
• GP206 (''aphA3'') & GP1442 (''aphA3'') , available in [SW|Stülke] lab
• BKE14140 (Δ[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT
• BKK14140 (Δ[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT

Expression vector

• for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP706, available in [SW|Stülke] lab

lacZ fusion

• pGP707 (in [protein|search|pAC5]), available in [SW|Stülke] lab, pGP711 (in [protein|search|pAC6]), available in [SW|Stülke] lab

Labs working on this gene/protein

• [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

References

• 17994626,23139400,11985717,10656796,12591885,14636591,16395550