rhaA [2019-07-09 11:27:43]

L-rhamnose isomerase

locus: BSU31180

pI: 5.35

mw: 48.48 kDa

protein length: 424 aa

gene length: 1275 bp

function: rhamnose utilization

product: L-rhamnose isomerase

essential: no

ec: 5.3.1.14

synonyms: yulE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.12|Utilization of rhamnose]

Gene

Coordinates: 3,197,933 → 3,199,207

The protein

Catalyzed reaction/ biological activity

• L-rhamnose = L-rhamnulose [Pubmed|26712933]

Protein family

• rhamnose isomerase family (according to Swiss-Prot)

Structure

• [PDB|3P14] (from B. halodurans, 78% identity) [pubmed|24925069]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|26712933,22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|26712933], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR]: repression, [Pubmed|26712933], in [regulon|F574142C1E61788FF8F4B61280AD4F87292D08DF|RhaR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|26712933], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

• genes: [gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]
  description: [Pubmed|22383849]

  regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B544 (yulE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1543 NBRP B. subtilis, Japan]
• BKE31180 (Δ[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA, downstream forward: _UP4_TAATAAAAAATCCGCCGCGG
• BKK31180 (Δ[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGGCTCTCTCCCTTTA, downstream forward: _UP4_TAATAAAAAATCCGCCGCGG

References

• 26712933,10960106,24391637,22383849,24925069