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pghL [2019-06-06 11:20:43]
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pghL [2019-06-06 11:20:43]

phage-derived gamma polyglutamic acid hydrolase, sporulation protein
locus
BSU17820
pI
6.92
mw
28.07 kDa
protein length
252 aa Sequence Blast
gene length
759 bp Sequence Blast
function
polyglutamic acid degradation
product
phage-derived gamma polyglutamic acid hydrolase
essential
no
synonyms
yndL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.4|Capsule biosynthesis and degradation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,915,221 → 1,915,979

    The protein

    Catalyzed reaction/ biological activity

  • hydrolysis of polyglutamic acid [Pubmed|26158264]

    • Protein family

  • UPF0714 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|PghZ], [protein|BA7D1D112E934B68073DFC1F76248ED6157240E3|PghB], [protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|PghC]

    • [SW|Domains]

  • DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]

    • Structure

  • [PDB|5ONJ] (complex with poly-γ-glutamate) [pubmed|30387270]
  • [PDB|3A9L] (from B. subtilis phage NIT1, 36% identity) [pubmed|22105902]
  • [PDB|6HRI] (native)
  • [PDB|6HRJ] (apo-form)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]
    description
    [Pubmed|16385044]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,16385044], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulation

  • expressed during [SW|sporulation] in the mother cell[Pubmed|16385044]

    • additional information

  • An [ncRNA|search|antisense RNA], '[protein|search|surA]', is predicted for '[protein|search|pghL]' [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-B113 (yndL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1112 NBRP B. subtilis, Japan]
  • BKE17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
  • BKK17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA

    • References

  • 16385044,20525796,26158264,27766092,22105902,30387270