pghL

phage-derived gamma polyglutamic acid hydrolase, sporulation protein

locus: BSU_17820

pI: 6.92

mw: 28.07 kDa

protein length: 252 aa

gene length: 759 bp

function: polyglutamic acid degradation

product: phage-derived gamma polyglutamic acid hydrolase

essential: no

synonyms: yndL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.4|Capsule biosynthesis and degradation]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,915,221 → 1,915,979

The protein

Catalyzed reaction/ biological activity

• hydrolysis of polyglutamic acid [Pubmed|26158264]

Protein family

• [SW|UPF0714 family] (according to UniProt)

Paralogous protein(s)

• [protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|PghZ], [protein|BA7D1D112E934B68073DFC1F76248ED6157240E3|PghB], [protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|PghC]

[SW|Domains]

• DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]

Structure

• [PDB|5ONJ] (complex with poly-γ-glutamate) [pubmed|30387270]
• [PDB|3A9L] (from B. subtilis phage NIT1, 36% identity) [pubmed|22105902]
• [PDB|6HRI] (native)
• [PDB|6HRJ] (apo-form)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]
  description: [Pubmed|16385044]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,16385044], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed during [SW|sporulation] in the mother cell[Pubmed|16385044]

  additional information

  • An [ncRNA|search|antisense RNA], '[protein|search|surA]', is predicted for '[protein|search|pghL]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-B113 (yndL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1112 NBRP B. subtilis, Japan]
• BKE17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
• BKK17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA

References

• 16385044,20525796,26158264,27766092,22105902,30387270