pghL

phage-derived gamma polyglutamic acid hydrolase, sporulation protein

Locus: BSU_17820

Isoelectric point: 6.92

Molecular weight: 28.07 kDa

Protein length: 252 aa

Gene length: 759 bp

Function: polyglutamic acid degradation

Product: phage-derived gamma polyglutamic acid hydrolase

Essential: no

Synonyms: yndL

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Capsule biosynthesis and degradation
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,915,221 → 1,915,979

The protein

Catalyzed reaction/ biological activity

• hydrolysis of polyglutamic acid PubMed

Protein family

• UPF0714 family (according to UniProt)

Paralogous protein(s)

• PghZ, PghB, PghC

Domains

• DUF867 x, [[gamma-PGA hydrolase domain]] PubMed

Structure

• 5ONJ (complex with poly-γ-glutamate) PubMed
• 3A9L (from B. subtilis phage NIT1, 36% identity) PubMed
• 6HRI (native)
• 6HRJ (apo-form)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pghL
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulation

  • expressed during sporulation in the mother cellPubMed

  Additional information

  • An [ncRNA|search|antisense RNA], 'surA', is predicted for 'pghL' PubMed

Biological materials

Mutant

• MGNA-B113 (yndL::erm), available at the NBRP B. subtilis, Japan
• BKE17820 (ΔpghL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
• BKK17820 (ΔpghL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAA

References

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