bsdC

phenolic acid decarboxylase

locus: BSU_03640

pI: 5.12

mw: 52.86 kDa

protein length: 473 aa

gene length: 1422 bp

function: resistance to salicylic acid

product: phenolic acid decarboxylase

essential: no

synonyms: yclC

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 413,157 → 414,578

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol [Pubmed|26658822]
• 4-hydroxybenzoate + H⁺ --> CO₂ + phenol (according to UniProt)
• 4-hydroxy-3-methoxybenzoate + H⁺ --> CO₂ + guaiacol (according to UniProt)

Protein family

• ubiD family (single memberm, according to UniProt)

Structure

• [PDB|5M1B] (from E. coli, 31% identity) [pubmed|28057757]

Expression and Regulation

Operons

• genes: [gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]-[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]-[gene|868DC9DE1C9C26C780F4CEAA4185640B5B6B781B|bsdD]
  description: [Pubmed|17295427]

  regulatory mechanism

  • [protein|28D03575FA24525C162E6C161631034568E89622|BsdA]: activation, [Pubmed|17295427], in [regulon|28D03575FA24525C162E6C161631034568E89622|BsdA regulon]

  regulation

  • induced by salicylic acid ([protein|search|BsdA]) [Pubmed|17295427]

Biological materials

Mutant

• MGNA-C062 (yclC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2060 NBRP B. subtilis, Japan]
• BKE03640 (Δ[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA, downstream forward: _UP4_AATAAATAAGGAAAGGATGT
• BKK03640 (Δ[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA, downstream forward: _UP4_AATAAATAAGGAAAGGATGT

References

• 15979273,17295427,26658822,28057757