bsdC

phenolic acid decarboxylase

Locus: BSU_03640

Isoelectric point: 5.12

Molecular weight: 52.86 kDa

Protein length: 473 aa

Gene length: 1422 bp

Function: resistance to salicylic acid

Product: phenolic acid decarboxylase

Essential: no

Synonyms: yclC

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 413,157 → 414,578

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol PubMed
• 4-hydroxybenzoate + H⁺ --> CO₂ + phenol (according to UniProt)
• 4-hydroxy-3-methoxybenzoate + H⁺ --> CO₂ + guaiacol (according to UniProt)

Protein family

• ubiD family (single memberm, according to UniProt)

Structure

• 5M1B (from E. coli, 31% identity) PubMed

Expression and Regulation

Operons

• Genes: bsdB-bsdC-bsdD
  Description: PubMed

  Regulatory mechanism

  • BsdA: activation, PubMed, in BsdA regulon

  Regulation

  • induced by salicylic acid (BsdA) PubMed

Biological materials

Mutant

• MGNA-C062 (yclC::erm), available at the NBRP B. subtilis, Japan
• BKE03640 (ΔbsdC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA, downstream forward: _UP4_AATAAATAAGGAAAGGATGT
• BKK03640 (ΔbsdC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGAAATCTTGATAAGCCA, downstream forward: _UP4_AATAAATAAGGAAAGGATGT

References

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