cwlK [2020-04-07 17:10:24]

peptidoglycan hydrolytic L,D-endopeptidase

locus: BSU_02810

pI: 5.66

mw: 18.97 kDa

protein length: 167 aa

gene length: 504 bp

function: cell wall turnover

product: peptidoglycan hydrolytic L,D-endopeptidase

essential: no

synonyms: ycdD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 303,804 → 304,307

The protein

Catalyzed reaction/ biological activity

• cleaves the peptide bond between L-Ala (position 1 in the peptioglycan peptide) and D-Glu (position 2) [Pubmed|18266855]

Protein family

• peptidase M15C family (single member, according to UniProt)

Structure

• [PDB|6AKV] (from Bacillus phage B4, correpsonds to aa 61 ... 164, 51% identity)

[SW|Localization]

• cell membrane [Pubmed|17588176]

Biological materials

Mutant

• BKE02810 (Δ[gene|FCE68CB323C7FDFE09B1F88EAEB95D045A189AEB|cwlK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGGTCTCACTTTCTC, downstream forward: _UP4_GAGATGATTCCTAACTAGAC
• BKK02810 (Δ[gene|FCE68CB323C7FDFE09B1F88EAEB95D045A189AEB|cwlK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGGTCTCACTTTCTC, downstream forward: _UP4_GAGATGATTCCTAACTAGAC

labs

• [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]

References

Reviews

• 18266855

Original publications

• 17588176