fapR [2019-07-01 10:31:13]

repressor of fatty acid synthetic genes

locus: BSU15880

pI: 5.39

mw: 21.26 kDa

protein length: 188 aa

gene length: 567 bp

function: regulation of fatty acid biosynthesis

product: transcriptional repressor

essential: no

synonyms: ylpC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 1,661,967 → 1,662,533

The protein

Catalyzed reaction/ biological activity

• FapR regulates the expression of at least 10 genes (the ''fap'' regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the ''fap'' regulon.

Protein family

• fapR family (according to Swiss-Prot)

Effectors of protein activity

• malonyl-CoA and malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP]]] act as the molecular inducer of the FapR regulon [Pubmed|20201588]

Structure

• [PDB|2F3X] (complex with effector), [PDB|2F41] [Pubmed|16932747]

Expression and Regulation

Operons

• genes: [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
  description: [Pubmed|19850612]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16932747], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

  regulation

  • ''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-B138 (ylpC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1137 NBRP B. subtilis, Japan]
• BKE15880 (Δ[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTAAACACCATCCGG, downstream forward: _UP4_AAACATTCATAAAGGTGGAG
• BKK15880 (Δ[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTAAACACCATCCGG, downstream forward: _UP4_AAACATTCATAAAGGTGGAG

References

Reviews

• 17919287,27766255,15802245,18372209

The [SW|FapR regulon]

• 12737802

Original publications

• 18384517,20201588,16932747,31113899