cmoI

required for the conversion of S-methyl-cysteine to cysteine

locus: BSU_29320

pI: 7.11

mw: 10.49 kDa

protein length: 93 aa

gene length: 282 bp

function: utilization of S-methyl-cysteine

product: unknown

essential: no

synonyms: ytnI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.1|Conversion of S-methyl cysteine to cysteine]

Gene

Coordinates: 3,003,049 → 3,003,330

Phenotypes of a mutant

• no growth with S-methyl cysteine [Pubmed|23944997]

The protein

Catalyzed reaction/ biological activity

• oxidation of S-methyl-cysteine [Pubmed|23944997]

Protein family

• glutaredoxin family (single member, according to UniProt)

[SW|Domains]

• Glutaredoxin domain (aa 1-93) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
  description: [Pubmed|15668000]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15272571], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|AscR]: activation, [Pubmed|16109943], in [regulon|741156D495BE3857683C8A0390764EAD83845ABC|AscR regulon]

  regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]

Biological materials

Mutant

• BKE29320 (Δ[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACTCATTCTTCTGTCCC, downstream forward: _UP4_GCACAGATAAAGGAGGCAAA
• BKK29320 (Δ[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACTCATTCTTCTGTCCC, downstream forward: _UP4_GCACAGATAAAGGAGGCAAA

References

• 16109943,15668000,15272571,23944997