SubtiBank SubtiBank
acuB
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acuB

unknown
Locus
BSU_29700
Isoelectric point
6.59
Molecular weight
24.21 kDa
Protein length
214 aa Sequence Blast
Gene length
645 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → Proteins of unknown function

    • Gene

    Coordinates
    3,040,751 → 3,041,395

    The protein

    Domains

  • 2 CBS domains (aa 7-66, aa 78-135) (according to UniProt)

    • Structure

  • 5AWE (from Thermus thermophilus, 30% identity) PubMed

    • Localization

  • Cytoplasm (Homogeneous) PubMed

    • Expression and Regulation

    Operons

    Genes
    acuA-acuB-acuC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • repressed by glucose (3.4-fold) (CcpA) PubMed

    • Additional information

  • the mRNA is quite stable (half-life 5 min) PubMed
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    Biological materials

    Mutant

  • BKE29700 (ΔacuB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
  • BKK29700 (ΔacuB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT

    • Expression vectors

  • pGP2922 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

    • References

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