sr1

small regulatory RNA controlling [protein|search|AhrC ]expression, regulatory peptide

locus: BSU_14629

pI: 4.00

mw: 4.00 kDa

function: control of arginine metabolism and glycolysis

product: small regulatory RNA, regulatory peptide

essential: no

synonyms: ykzW,sr1

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.4|Utilization of arginine/ ornithine]
• [category|SW 6|Groups of genes] → [category|SW 6.9|NcRNA] → [category|SW 6.9.5|Regulatory RNAs]

Gene

Coordinates: 1,534,120 → 1,534,239

RNA

Catalyzed reaction/ biological activity

• hybridizes to ''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]'' mRNA and inhibits [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC] translation [Pubmed|17020585]

[SW|Interactions]

• [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA-[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA [Pubmed|20444087]
• [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA]-[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA to promote base pairing between the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA and the [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA and to control arginine metabolism [pubmed|31043113]

Additional information

• Information on the structure of RNA can be found in [Pubmed|17020585]

Expression and Regulation

Operons

• genes: [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]
  description: [Pubmed|16164558]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16164558], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN]: repression, in [regulon|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN regulon]

  regulation

  • Repressed in the presence of glucose by [protein|search|CcpN] [Pubmed|16164558]

Biological materials

Mutant

• 168 ykzW::cat, available in [SW|Sabine Brantl]'s lab
• GP2210 (''[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]''::''cat''), available in [SW|Jörg Stülke]'s lab
• GP2215 (''[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
• GP2216 (''[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]''::''phleo''), available in [SW|Jörg Stülke]'s lab
• BKE14629 (Δ[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14629 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTATTCCCCATTTC, downstream forward: _UP4_TAAAAAAAAGCATGCGGCTT
• BKK14629 (Δ[gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14629 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTATTCCCCATTTC, downstream forward: _UP4_TAAAAAAAAGCATGCGGCTT

labs

• [SW|Sabine Brantl], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]

References

Reviews

• 27750368,30191807,32850966

Original Publications

• 16164558,17020585,17576690,20444087,23034808,27449348,28818119,31043113

The peptide

Catalyzed reaction/ biological activity:

• the peptide controls the stability of the ''[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]'' mRNA [Pubmed|20444087]

Protein family

• the peptide is conserved in bacilli

[SW|Interactions]

• [protein|EB6512177418B1601C6641FB2DEE99C2CD10E671|GapA]-[protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|Sr1] [Pubmed|20444087]