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dprA
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dprA

conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex
Locus
BSU_16110
Isoelectric point
9.76
Molecular weight
32.78 kDa
Protein length
297 aa Sequence Blast
Gene length
894 bp Sequence Blast
Product
recombination mediator protein
Essential
no
Synonyms
smf

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,682,580 → 1,683,473

Phenotypes of a mutant

  • strongly impaired genetic recombination PubMed
  • recO dprA double mutants have a strongly reduced chromosomal transformation rate PubMed
  • loss of plasmid transformation PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • binds ssDNA, promotes displacement of SsbA and SsbB from ssDNA PubMed
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed
  • RecA-ATP in concert with DprA and SsbA catalyzes DNA strand exchange, with SsbB as an accessory factor PubMed
  • the SsbA-DprA mediator loads RecA onto any fragmented linear SPP1 ssDNA PubMed
  • anneals SsbA- or SsbB-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates PubMed
  • DprA, RecO or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication PubMed
  • Protein family

  • DprA/Smf family (single member, according to UniProt)
  • Structure

  • 3UQZ (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) PubMed
  • Localization

  • transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed by glucose (2.4-fold) (CcpA) PubMed
  • expression is heterogeneous PubMed
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    Additional information

  • expressed under conditions that trigger genetic competence (ComK) PubMed
  • Biological materials

    Mutant

  • BKE16110 (ΔdprA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
  • BKK16110 (ΔdprA::kan trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
  • Labs working on this gene/protein

  • Peter Graumann, Freiburg University, Germany homepage
  • References

    Reviews

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    Original publications

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