SubtiBank SubtiBank
ytdA [2019-08-09 08:14:20]
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ytdA [2019-08-09 08:14:20]

similar to UTP-glucose-1-phosphate uridylyltransferase
locus
BSU_30850
pI
5.91
mw
30.03 kDa
protein length
272 aa Sequence Blast
gene length
819 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.7.7.9
synonyms
yzwA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,154,735 → 3,155,553

    The protein

    Catalyzed reaction/ biological activity

  • UTP alpha-D-glucose 1-phosphate = diphosphate UDP-glucose (according to Swiss-Prot)

    • Protein family

  • UDPGP type 2 family (with [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB] and [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|YngB], according to UniProt)

    • Paralogous protein(s)

  • [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|YngB], [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB]

    • Structure

  • [PDB|3JUJ] (UDP-glucose pyrophosphorylase from ''Helicobacter pylori'', 43% identity) [Pubmed|20238176]

    • [SW|Localization]

  • outer spore coat [Pubmed|26577401]

    • Expression and Regulation

    Operons

    genes
    [gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulation

  • expressed late during [SW|sporulation] in the mother cell ([SW|SigK]) [Pubmed|26577401]
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    Biological materials

    Mutant

  • MGNA-A281 (ytdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/281 NBRP B. subtilis, Japan]
  • BKE30850 (Δ[gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC, downstream forward: _UP4_AAGAACCCCCAATAATTGGG
  • BKK30850 (Δ[gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC, downstream forward: _UP4_AAGAACCCCCAATAATTGGG

    • References

  • 26577401,20238176,22383849