hutG

formiminoglutamate hydrolase

Locus: BSU_39380

Isoelectric point: 5.44

Molecular weight: 34.91 kDa

Protein length: 319 aa

Gene length: 960 bp

Function: histidine utilization

Product: formiminoglutamate hydrolase

Essential: no

E.C.: 3.5.3.8

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of histidine

Gene

Coordinates: 4,046,503 → 4,047,462

The protein

Catalyzed reaction/ biological activity

• H₂O + N-formimidoyl-L-glutamate --> formamide + L-glutamate (according to UniProt)

Protein family

• arginase family (with RocF and SpeB, according to UniProt)

Structure

• 3M1R

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • CodY: repression, PubMed, in CodY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in HutP regulon

  Regulation

  • induced by histidine (HutP) PubMed

Biological materials

Mutant

• BKE39380 (ΔhutG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCGGCCTCTCTGAGAAAAG, downstream forward: _UP4_TGATCTTCTGGCAGGTTGTA
• BKK39380 (ΔhutG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCCGGCCTCTCTGAGAAAAG, downstream forward: _UP4_TGATCTTCTGGCAGGTTGTA

References

Reviews

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Original publications

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