asnS [2019-03-30 18:21:30]

asparagyl-tRNA synthetase

locus: BSU22360

pI: 5.12

mw: 48.92 kDa

protein length: 430 aa

gene length: 1293 bp

function: translation

product: asparagyl-tRNA synthetase

essential: no

ec: 6.1.1.22

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]

Gene

Coordinates: 2,346,224 → 2,347,516

Phenotypes of a mutant

• essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn) (according to Swiss-Prot)

Structure

• [PDB|1X56] (from ''Pyrococcus horikoshii'', 42% identity) [Pubmed|16753178]

Expression and Regulation

Operons

• genes: [gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• GP2934 (10 bp deletion at bp 1013) (no resistance) available in [SW|Jörg Stülke]'s lab
• BKE22360 (Δ[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATATTTCCCTCCAAAA, downstream forward: _UP4_TAATACATTCAAATGAAACA
• BKK22360 (Δ[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATATTTCCCTCCAAAA, downstream forward: _UP4_TAATACATTCAAATGAAACA

References

• 16753178,28189581