mmgB [2017-05-11 18:33:15]

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

locus: BSU24160

pI: 6.60

mw: 26.92 kDa

protein length: 244 aa

gene length: 732 bp

function: mother cell metabolism

product: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

essential: no

ec: 1.1.1.157

synonyms: yqiM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 2,511,973 → 2,512,836

The protein

Catalyzed reaction/ biological activity

• (S)-3-hydroxybutanoyl-CoA + NADP⁺ = 3-acetoacetyl-CoA + NADPH (according to Swiss-Prot)

Protein family

• 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)

Structure

• [PDB|4R1N] (from Clostridium butyricum, 56% identity) [pubmed|25112316]

Expression and Regulation

Operons

• genes: [gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
  description: [Pubmed|8759838]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8759838], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|15699190,8759838]
  • strongly expressed during oligotrophic growth [pubmed|30792386]

References

• 8759838,15699190,25112316