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cdaR [Mon Nov 14 2016 13:03:26 GMT+0100 (CET)]
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cdaR [Mon Nov 14 2016 13:03:26 GMT+0100 (CET)]

effector protein controlling CdaA diadenylate cyclase activity
locus
BSU01760
pI
4.00
mw
52.00 kDa
function
regulation of c-di-AMP synthesis
product
effector protein controlling CdaA diadenylate cyclase activity
essential
no
synonyms
ybbR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]

    • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides], [SW|membrane proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    197,027 -> 198,478

    The protein

    Catalyzed reaction/ biological activity

  • stimulates [protein|search|CdaA] activity upon protein-protein interaction [Pubmed|23192352]

    • Structure

  • [PDB|2KPU] (one YbbR domain, from ''Desulfitobacterium hafniense'') [Pubmed|21154411]

    • [SW|Localization]

  • cell membrane, oriented towards the outside [Pubmed|26240071]

    • [SW|Interactions]

  • [protein|search|CdaA]-[protein|search|CdaR], to control [protein|search|CdaA] activity [Pubmed|26240071,23192352]

    • Expression and Regulation

    Operon

  • ''[protein|search|cdaA]-[protein|search|cdaR]-[protein|search|glmM]-[protein|search|glmS]'' [Pubmed|23192352]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|22211522]

    • Additional information

  • the '[protein|search|cdaA]-[protein|search|cdaR]' part of the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

    • Biological materials

    Mutant

  • GP999 (''[protein|search|cdaR]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP985 (''[protein|search|cdaA]''-''[protein|search|cdaR]''::''cat''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • IPTG inducible expression of Strep-''cdaR'' in ''E. coli'': pGP2565 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • IPTG inducible expression of His-''cdaR'' in ''E. coli'': pGP2557 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab
  • GP2031: ''cdaR-Strep aphA3'', chromosomal expression in B. subtilis, for [protein|search|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab. Respective plasmids: pGP1989 and pGP1992.

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 25869574

    • Original publications

  • 12884008,16236721,26240071,23192352,22211522,21154411,26025048,26527648