yaaA [2019-03-19 18:53:35]

ribosome assembly factor, participates in the assembly of the 50S subunit of the ribosome

locus: BSU00030

pI: 4.33

mw: 7.79 kDa

protein length: 71 aa

gene length: 216 bp

function: assembly of the 50S subunit of the ribosome

product: ribosome assembly factor

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]

Gene

Coordinates: 3,206 → 3,421

Phenotypes of a mutant

• enhanced expression suppresses the defects of a temperature-sensitive ''[gene|9445AC486A2B0A025030B275C789D227D5694145|rplB]'' mutant [Pubmed|24637032]

Expression and Regulation

Operons

• genes: [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B879 (yaaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1878 NBRP B. subtilis, Japan]
• BKE00030 (Δ[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGACCTCTTTCA, downstream forward: _UP4_GTCAATTAAAGCGGGTGACA
• BKK00030 (Δ[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGACCTCTTTCA, downstream forward: _UP4_GTCAATTAAAGCGGGTGACA

References

• 2987848,24637032