yqhY [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

unknown

locus: BSU24330

pI: 4.41

mw: 14.00 kDa

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]

[SW|Categories] containing this gene/protein

• [SW|proteins of unknown function]

Gene

Coordinates on the chromosome (coding sequence): 2,529,926 -> 2,530,333

The protein

Protein family

• asp23 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|YloU]

Expression and Regulation

Operon

• ''[protein|search|accB]-[protein|search|accC]-[protein|search|yqhY]'' [Pubmed|7592499]

Biological materials

Mutant

• GP1468 (''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
• GP1765 (''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]

Expression vector

• GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
• pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
• pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1471 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1481 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

• 17114254,7592499,23420519,24178028