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licT [Thu Nov 03 2016 15:13:52 GMT+0100 (CET)]
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licT [Thu Nov 03 2016 15:13:52 GMT+0100 (CET)]

transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene
locus
BSU39080
pI
5.00
mw
32.00 kDa
function
control of beta-glucan and beta-glucoside utilization
product
transcriptional antiterminator (BglG family)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,012,866 -> 4,013,699

    Phenotypes of a mutant

  • no expression of the ''[protein|search|bglP]-[protein|search|bglH]'' operon

    • The protein

    Catalyzed reaction/ biological activity

  • binding to the mRNAs of ''[protein|search|bglS]'' and the ''[protein|search|bglP]-[protein|search|bglH]'' operon, causes transcription antitermination (in presence of salicin and absence of glucose)

    • Protein family

  • [PRD-containing transcription factors|search|transcriptional antiterminator] BglG family of antiterminators (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|SacY], [protein|search|GlcT], [protein|search|SacT]

    • [SW|Domains]

  • N-terminal RNA binding domain [Pubmed|10610766]
  • 2 x [SW|PRD] ([SW|PTS] regulation domains) [Pubmed|11447120]

    • Modification

  • phosphorylation at His-100 in [SW|PRD]-1 by phosphorylated [protein|search|BglP], inhibits LicT antitermination activity
  • phosphorylation at His-207 and/or His-269 in [SW|PRD]-2 by His-P-[ptsH|search|HPr], stimulates LicT antitermination activity

    • Structure

  • [PDB|1L1C] (complex with RAT)
  • [PDB|1TLV] ([SW|PRD]s)

    • [SW|Localization]

  • cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin [Pubmed|23475962]

    • [SW|Interactions]

  • [protein|search|PtsH]-[protein|search|LicT], [protein|search|BglP]-[protein|search|LicT]

    • Expression and Regulation

    Operon

  • ''[protein|search|licT]-[protein|search|bglS]'' [Pubmed|8606172]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8606172]

    • Biological materials

    Mutant

  • GP427 (licTS, erm), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP165, available in [SW|Jörg Stülke]'s lab
  • for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP315, available in [SW|Jörg Stülke]'s lab
  • for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP572, available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1225 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1221 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • [SW|Josef Deutscher], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • [SW|Michael Hecker], Greifswald, Germany [http://www.mikrobiologie.uni-greifswald.de/index.php?id=20 Homepage]

    • References

    Reviews

  • 9663674,18086213

    • Original publications

  • 8606172,23475962,11447120,10610766,7559347,8626332,10048041,11447120,11580842,11733988,12169607,12398354,15699035,17074746,21278164,21335451