licT [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene

Locus: BSU39080

Isoelectric point: 5.00

Molecular weight: 32.00 kDa

Function: control of beta-glucan and beta-glucoside utilization

Product: transcriptional antiterminator (BglG family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of beta-glucosides
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
• Information processing → Regulation of gene expression → Transcription factors and their control → PRD-type regulators
• Information processing → Regulation of gene expression → RNA binding regulators
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Categories containing this gene/protein

• utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

Gene

Coordinates on the chromosome (coding sequence): 4,012,866 -> 4,013,699

Phenotypes of a mutant

• no expression of the bglP-bglH operon

The protein

Catalyzed reaction/ biological activity

• binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)

Protein family

• [PRD-containing transcription factors|search|transcriptional antiterminator] BglG family of antiterminators (according to Swiss-Prot)

Paralogous protein(s)

• SacY, GlcT, SacT

Domains

• N-terminal RNA binding domain PubMed
• 2 x PRD (PTS regulation domains) PubMed

Modification

• phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
• phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-[ptsH|search|HPr], stimulates LicT antitermination activity

Structure

• 1L1C (complex with RAT)
• 1TLV (PRDs)

Localization

• cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin PubMed

Interactions

• PtsH-LicT, BglP-LicT

Expression and Regulation

Operon

• licT-bglS PubMed

Sigma factor

• SigA PubMed

Biological materials

Mutant

• GP427 (licTS, erm), available in Jörg Stülke's lab

Expression vector

• for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165, available in Jörg Stülke's lab
• for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315, available in Jörg Stülke's lab
• for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572, available in Jörg Stülke's lab

GFP fusion

• GP1225 (spc, based on pGP1870), available in Jörg Stülke's lab

FLAG-tag construct

• GP1221 (spc, based on pGP1331), available in Jörg Stülke's lab

Labs working on this gene/protein

• Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
• Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
• Michael Hecker, Greifswald, Germany Homepage

References

Reviews

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Original publications

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