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nasD [Mon Aug 31 2015 13:52:20 GMT+0200 (CEST)]
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nasD [Mon Aug 31 2015 13:52:20 GMT+0200 (CEST)]

assimilatory nitrite reductase (subunit)
locus
BSU03300
pI
4.95
mw
88.00 kDa
function
utilization of nitrite as nitrogen source
product
assimilatory nitrite reductase (subunit)
essential
no
ec
1.7.1.4
synonyms
nasBC, nirB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.1|Utilization of nitrate/ nitrite]
    • syns
    nasBC, nirB

    [SW|Categories] containing this gene/protein

  • [SW|respiration], [SW|utilization of nitrogen sources other than amino acids]

    • This gene is a member of the following [SW|regulons]

  • [SW|NsrR regulon], [SW|ResD regulon], [SW|TnrA regulon]

    • The protein

    Catalyzed reaction/ biological activity

  • Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

    • Protein family

  • nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|NasB]

    • [SW|Cofactors]

  • Fe-S cluster [Pubmed|11289299]
  • FAD [Pubmed|11289299]

    • [SW|Interactions]

  • [protein|search|NasD]-[protein|search|NasE] [Pubmed|11289299]

    • Expression and Regulation

    Operon

  • ''[protein|search|nasB]-[protein|search|nasC]-[protein|search|nasD]-[protein|search|nasE]-[protein|search|nasF]'' [Pubmed|7836289]
  • ''[protein|search|nasD]-[protein|search|nasE]-[protein|search|nasF]'' [Pubmed|9765565]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7836289]

    • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
  • ''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
  • induced by nitric oxide under anaerobic conditions ([protein|search|NsrR]) [Pubmed|16885456]

    • Regulatory mechanism

  • [protein|search|TnrA]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
  • [protein|search|ResD]: transcription activation [Pubmed|10972836]
  • [protein|search|NsrR]: transcription repression [Pubmed|16885456]

    • Biological materials

    Mutant

  • 1A973 ( ''nasD''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A973&Search=1A973 BGSC]

    • References

    Reviews

  • 22103536,11289299

    • Original publications

  • 12823818,7868621,8799114,9765565,10972836,16885456,21091510,24214949,25755103