cotD

spore coat protein (inner)

Locus: BSU_22200

Isoelectric point: 7.37

Molecular weight: 8.70 kDa

Protein length: 75 aa

Gene length: 228 bp

Function: resistance of the spore

Product: spore coat protein (inner)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class VI

Gene

Coordinates: 2,332,784 → 2,333,011

The protein

Localization

• inner spore coat PubMed, localization depends on SafA PubMed

Expression and Regulation

Operons

• Genes: cotD
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon
  • GerE: activation, PubMed, in GerE regulon

  Regulation

  • expressed late during sporulation in the mother cell (SigK, SpoIIID, GerE) PubMed

• Genes: mrfA-mrfB-cotD
  Description: PubMed

Biological materials

Mutant

• 1S104 ( cotD::cat), PubMed, available at BGSC
• BKE22200 (ΔcotD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAATCCCCTTTCC, downstream forward: _UP4_TAGTATGGAAAACGTGCAGC
• BKK22200 (ΔcotD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAATCCCCTTTCC, downstream forward: _UP4_TAGTATGGAAAACGTGCAGC

References

Reviews

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Original publications

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