thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of spore killing factor
function
maturation of spore killing factor
product
thioether bond forming radical SAM enzyme
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins][category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1][SW|Categories] containing this gene/protein
[SW|toxins, antitoxins and immunity against toxins], [SW|prophage 1]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|PhoP regulon], [SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
214,175 -> 215,407
The protein
Catalyzed reaction/ biological activity
generates a thioether bond in [protein|search|SkfA], which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 [Pubmed|23282011]Protein family
moaA/nifB/pqqE family (according to Swiss-Prot)[SW|Cofactors]
contains two [4Fe-4S] clusters [Pubmed|23282011][SW|Interactions]
[protein|search|SkfA]-[protein|search|SkfB] [Pubmed|23282011]Expression and Regulation
Operon
''[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]'' [Pubmed|16816204][SW|Sigma factor]
[protein|search|SigA] [Pubmed|16452424]Regulation
expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]induced by phosphate starvation ([protein|search|PhoP]) [Pubmed|16816204]Regulatory mechanism
[SW|Spo0A]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed][protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed][protein|search|PhoP]: transcription activation [Pubmed|16816204]Additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|search|skfA]'-specific transcript, and a weaker 6.5 kb '[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]' transcript. The '[protein|search|skfA]' transcript is stabilized by a stem-loop structure in the '[protein|search|skfA]-[protein|search|skfB]' intergenic region. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]References
