skfB

thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]

locus: BSU_01920

pI: 4.93

mw: 45.59 kDa

protein length: 410 aa

gene length: 1233 bp

function: maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]

product: AdoMet radical sactisynthase

essential: no

synonyms: ybcP,ybcQ

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]

Gene

Coordinates: 214,175 → 215,407

The protein

Catalyzed reaction/ biological activity

• generates a thioether bond in [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA], which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 [Pubmed|23282011]

Protein family

• [SW|Radical SAM superfamily] (according to UniProt)

[SW|Cofactors]

• [4Fe-4S] and [2Fe-2S] clusters [Pubmed|30217813,23282011]

Structure

• [PDB|6EFN] [Pubmed|30217813]

Expression and Regulation

Operons

• genes: [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
  description: [Pubmed|16816204]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452424], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|15687200,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16816204], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

  additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA]'-specific transcript, and a weaker 6.5 kb [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH] transcript

Biological materials

Mutant

• MGNA-C513 (ybcQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2511 NBRP B. subtilis, Japan]
• BKE01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG, downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
• BKK01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG, downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA

References

Reviews

• 20955377

Research papers

• 23282011,14651647,15687200,17720793,16816204,16452424,30217813