rplK

ribosomal protein

Locus: BSU_01020

Isoelectric point: 9.72

Molecular weight: 14.78 kDa

Protein length: 141 aa

Gene length: 426 bp

Function: translation

Product: ribosomal protein L11 (BL11)

Essential: no

Synonyms: relC,tsp

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → Newly identified competence genes
• Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Universally conserved proteins

Gene

Coordinates: 118,591 → 119,016

Phenotypes of a mutant

• poor growth PubMed
• non-transformable PubMed

The protein

Protein family

• Universal ribosomal protein uL11 family (single member, according to UniProt)

Modification

• phosphorylated on Arg-94 PubMed

Structure

• 1FOX (C-terminal domain, Geobacillus stearothermophilus)
• 2FOW (RNA binding domain in complex with RNA, Geobacillus stearothermophilus)
• 3J9W (the ribosome) PubMed

Localization

• ribosome (according to UniProt)

Expression and Regulation

Operons

• Genes: rplK-rplA-BSU_misc_RNA_3-rplJ-rplL
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

Biological materials

Mutant

• BKE01020 (ΔrplK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT
• BKK01020 (ΔrplK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT

References

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