SubtiBank SubtiBank
metP [2018-05-30 12:50:47]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

metP [2018-05-30 12:50:47]

methionine ABC transporter, permease
locus
BSU32740
pI
6.62
mw
23.61 kDa
protein length
222 aa Sequence Blast
gene length
666 bp Sequence Blast
function
methionine uptake
product
methionine ABC transporter, permease
essential
no
synonyms
yusB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,362,605 → 3,363,273

    The protein

    Structure

  • [PDB|3DHW] (the [protein|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|MetN]-[protein|F94070DD039A422055FFC35087FB87765F22E156|MetP] complex from ''E. coli'', 44% identity) [Pubmed|18621668]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|D7254B7F31D3F4CD18172AFB0F526CAE5195BDB6|metN]-[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]-[gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ]
    description
    [Pubmed|10094622]

    regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination/ antitermination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|S-box|S-box]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]
  • the mRNA is processed between [gene|F94070DD039A422055FFC35087FB87765F22E156|metP] and [gene|0481A9C134A6EEC6F111AD0C03E2E345D6A315A0|metQ] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A600 (yusB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/600 NBRP B. subtilis, Japan]
  • 1A797 ( ''metP''::''cat''), [Pubmed|14990259], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
  • BKE32740 (Δ[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCAAACATTCGTGATCAC, downstream forward: _UP4_GACAAACGATAAGGGAGGAT
  • BKK32740 (Δ[gene|F94070DD039A422055FFC35087FB87765F22E156|metP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCAAACATTCGTGATCAC, downstream forward: _UP4_GACAAACGATAAGGGAGGAT

    • References

  • 10092453,14990259,12618455,10094622,18039762,18621668