xynP [2019-06-06 13:15:07]

beta-xyloside permease

locus: BSU17570

pI: 8.58

mw: 51.37 kDa

protein length: 463 aa

gene length: 1392 bp

function: xylan utilization

product: beta-xyloside permease

essential: no

synonyms: ynaJ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.3|Carbohydrate transporter]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.5|Utilization of xylan/ xylose]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,887,352 → 1,888,743

The protein

Protein family

• [SW|sodium:galactoside symporter (TC 2.A.2) family] (according to UniProt)

Paralogous protein(s)

• [protein|B13F7C35FBD55C674E5B19836D32279E7C1A5520|GutP], [protein|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|YjmB]

Structure

• [PDB|4M64] (melibiose permease from Salmonella typhimurium, 31% identity) [pubmed|24389923]

Expression and Regulation

Operons

• genes: [gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]-[gene|19CD9BF216953613CD2E0DFB99D4C6E92AAFAE5D|xynB]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9973552], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|9973552], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|AF4395D134485290F4AA307B48494FED39E52CD7|XylR]: repression, [Pubmed|9973552], in [regulon|AF4395D134485290F4AA307B48494FED39E52CD7|XylR regulon]

  regulation

  • carbon catabolite repression ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|9973552]
  • induction by xylose ([protein|AF4395D134485290F4AA307B48494FED39E52CD7|XylR]) [Pubmed|9973552]

Biological materials

Mutant

• MGNA-B107 (ynaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1106 NBRP B. subtilis, Japan]
• BKE17570 (Δ[gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG, downstream forward: _UP4_TAAAAAAGAAAATAAACTGA
• BKK17570 (Δ[gene|F92925E2FDCFE6DDA3E87C7B541FE6A6CE5AABB8|xynP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG, downstream forward: _UP4_TAAAAAAGAAAATAAACTGA

lacZ fusion

• pGP417 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

References

Carbon catabolite repression

• 18757537,9973552,22001508,22900538,24389923