xynP

beta-xyloside permease

Locus: BSU_17570

Isoelectric point: 8.58

Molecular weight: 51.37 kDa

Protein length: 463 aa

Gene length: 1392 bp

Function: xylan utilization

Product: beta-xyloside permease

Essential: no

Synonyms: ynaJ

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Carbohydrate transporter
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of xylan/ xylose
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,887,352 → 1,888,743

The protein

Protein family

• sodium:galactoside symporter (TC 2.A.2) family (according to UniProt)

Paralogous protein(s)

• GutP, YjmB

Structure

• 4M64 (melibiose permease from Salmonella typhimurium, 31% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: xynP-xynB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • XylR: repression, PubMed, in XylR regulon

  Regulation

  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

Biological materials

Mutant

• MGNA-B107 (ynaJ::erm), available at the NBRP B. subtilis, Japan
• BKE17570 (ΔxynP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG, downstream forward: _UP4_TAAAAAAGAAAATAAACTGA
• BKK17570 (ΔxynP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCTCCCCTTTTCG, downstream forward: _UP4_TAAAAAAGAAAATAAACTGA

LacZ fusion

• pGP417 (in pAC7), available in Jörg Stülke's lab

References

Carbon catabolite repression

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