bacG

short chain reductase involved in bacilysin synthesis

locus: BSU_37680

pI: 6.60

mw: 27.87 kDa

protein length: 259 aa

gene length: 780 bp

function: biosynthesis of the antibiotic bacilysin

product: short chain reductase

essential: no

synonyms: ipa-86r,ywfH

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 3,867,493 → 3,868,272

The protein

Catalyzed reaction/ biological activity

• BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate [Pubmed|20052993]
• stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP [Pubmed|23519407]

Protein family

• [SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

[SW|Cofactors]

• NADPH [Pubmed|23519407]

Structure

• [PDB|3U49] [Pubmed|23519407]

Expression and Regulation

Operons

• genes: [gene|F8FF994BF2FDFB27DE63C1F8FB75DAB7E90FC78C|bacG]
  description: [Pubmed|12372825]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12372825], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by casamino acids [Pubmed|12107147]

Biological materials

Mutant

• MGNA-A515 (ywfH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/515 NBRP B. subtilis, Japan]
• BKE37680 (Δ[gene|F8FF994BF2FDFB27DE63C1F8FB75DAB7E90FC78C|bacG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAACTGCACCCCTTTG, downstream forward: _UP4_AGCATATAAAAACATCCCGC
• BKK37680 (Δ[gene|F8FF994BF2FDFB27DE63C1F8FB75DAB7E90FC78C|bacG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAACTGCACCCCTTTG, downstream forward: _UP4_AGCATATAAAAACATCCCGC

References

• 12372825,12107147,20052993,21948839,23519407