maeN [2019-07-10 09:07:20]

Na⁺/malate symporter

locus: BSU31580

pI: 8.41

mw: 47.66 kDa

protein length: 448 aa

gene length: 1347 bp

function: malate uptake

product: Na⁺/malate symporter

essential: no

synonyms: yufR

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,244,770 → 3,246,116

The protein

Protein family

• sodium:citrate (SCF) symporter family 2-hydroxycarboxylate transporter family (together with [protein|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|CimH]) (according to UniProt)

Paralogous protein(s)

• [protein|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|CimH]

Structure

• [PDB|5X9R] (from Klebsiella pneumoniae, 34% identity) [pubmed|28566738]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]

  regulation

  • induced by malate ([protein|search|MalR])

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B556 (yufR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1555 NBRP B. subtilis, Japan]
• 1A642 (''maeN''::Tn917, erm) (available from the [http://www.bgsc.org/ BGSC] or in [SW|Jörg Stülke]'s lab)
• 1A642 ( ''maeN''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
• GP1449 (''erm''), available in [SW|Stülke] lab
• BKE31580 (Δ[gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
• BKK31580 (Δ[gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT

Expression vectors

• expression of native ''maeN'' in ''B. subtilis'': pGP1951 (in [SW|pBQ200]), available in [SW|Stülke] lab

References

Reviews

• 16339740

Original publications

• 19087206,12949159,12949159,21815947,28566738