maeN [2019-07-10 09:07:20]

Na⁺/malate symporter

Locus: BSU31580

Isoelectric point: 8.41

Molecular weight: 47.66 kDa

Protein length: 448 aa

Gene length: 1347 bp

Function: malate uptake

Product: Na⁺/malate symporter

Essential: no

Synonyms: yufR

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,244,770 → 3,246,116

The protein

Protein family

• sodium:citrate (SCF) symporter family 2-hydroxycarboxylate transporter family (together with CimH) (according to UniProt)

Paralogous protein(s)

• CimH

Structure

• 5X9R (from Klebsiella pneumoniae, 34% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: maeN
  Description: PubMed

  Regulatory mechanism

  • MalR: activation, in MalR regulon

  Regulation

  • induced by malate (MalR)

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B556 (yufR::erm), available at the NBRP B. subtilis, Japan
• 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
• 1A642 ( maeN::erm), PubMed, available at BGSC
• GP1449 (erm), available in Stülke lab
• BKE31580 (ΔmaeN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
• BKK31580 (ΔmaeN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT

Expression vectors

• expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab

References

Reviews

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Original publications

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