SubtiBank SubtiBank
maeN [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

maeN [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

Na+/malate symporter
locus
BSU31580
pI
8.41
mw
47.00 kDa
function
malate uptake
product
Na+/malate symporter
essential
no
synonyms
yufR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|utilization of specific carbon sources], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|MalR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,244,770 -> 3,246,116

    The protein

    Protein family

  • sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family

    • Paralogous protein(s)

  • [protein|search|CimH]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|maeN]'' [Pubmed|21815947]

    • Regulation

  • induced by malate ([protein|search|MalR])

    • Regulatory mechanism

  • [protein|search|MalR]: transcription activation

    • Additional information

  • the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] [PubMed|21815947]

    • Biological materials

    Mutant

  • 1A642 (''maeN''::Tn917, erm) (available from the [http://www.bgsc.org/ BGSC] or in [SW|Jörg Stülke]'s lab)
  • 1A642 ( ''maeN''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
  • GP1449 (''erm''), available in [SW|Stülke] lab

    • Expression vector

  • expression of native ''maeN'' in ''B. subtilis'': pGP1951 (in [SW|pBQ200]), available in [SW|Stülke] lab

    • References

    Reviews

  • 16339740

    • Original publications

  • 19087206,12949159,12949159,21815947