SubtiBank SubtiBank
maeN [2018-02-13T15:04:36.000Z]
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maeN [2018-02-13T15:04:36.000Z]

Na+/malate symporter
Locus
BSU31580
Isoelectric point
8.41
Molecular weight
47.66 kDa
Protein length
448 aa Sequence Blast
Gene length
1344 bp Sequence Blast
Function
malate uptake
Product
Na+/malate symporter
Essential
no
Synonyms
yufR

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,244,770 → 3,246,116

The protein

Protein family

  • sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
  • Paralogous protein(s)

    Localization

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • MalR: activation, in MalR regulon
  • Regulation

  • induced by malate (MalR)
  • Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B556 (yufR::erm), available at the NBRP B. subtilis, Japan
  • 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
  • 1A642 ( maeN::erm), PubMed, available at BGSC
  • GP1449 (erm), available in Stülke lab
  • BKE31580 (ΔmaeN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
  • BKK31580 (ΔmaeN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
  • Expression vector

  • expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab
  • References

    Reviews

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    Original publications

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