You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
fadM [2019-05-10 14:20:05]
fatty acid degradation, similar to proline dehydrogenase
function
fatty acid degradation
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]Gene
Coordinates
3,372,740 → 3,373,648
The protein
Catalyzed reaction/ biological activity
L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)Protein family
proline dehydrogenase family (according to Swiss-Prot)Paralogous protein(s)
[protein|1F548515402950572E7212901729B2480432D1B9|PutB] Structure
[PDB|4H6Q] (from Deinococcus radiodurans, 48% identity) [pubmed|23151026]Expression and Regulation
Operons
genes
[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]-[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
description
[Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon][protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: activation, [Pubmed|12817086], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]regulation
''[protein|search|fadM]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]view in new tabBiological materials
Mutant
MGNA-A598 (yusM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/598 NBRP B. subtilis, Japan]BKE32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGCBKK32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG, downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGCReferences
17189250,12817086,23151026