yfkM

glyoxalase III-like enzyme, general stress protein, survival of salt, paraquat and ethanol stresses

locus: BSU_07850

pI: 4.72

mw: 18.72 kDa

protein length: 172 aa

gene length: 519 bp

function: detoxification of methylglyoxal

product: glyoxalase III-like enzyme

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 859,745 → 860,263

The protein

Catalyzed reaction/ biological activity

• methylglyoxal -→ D-lactate [Pubmed|24330391]

Protein family

• [SW|peptidase C56 family] (according to UniProt)

Paralogous protein(s)

• [protein|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|YraA]

[SW|Domains]

• [SW|PfpI endopeptidase domain] (aa 3-171) (according to UniProt)

Structure

• [PDB|1OI4] (from E. coli, 64% identity)

Expression and Regulation

Operons

• genes: [gene|F88F0153CAFC1E51F2586F733AFDF22320642151|yfkM]
  description: [Pubmed|12354229]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-C264 (yfkM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2262 NBRP B. subtilis, Japan]
• BKE07850 (Δ[gene|F88F0153CAFC1E51F2586F733AFDF22320642151|yfkM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCATAACCTCCCGC, downstream forward: _UP4_TAAGAAAAAACGGACGCTCT
• BKK07850 (Δ[gene|F88F0153CAFC1E51F2586F733AFDF22320642151|yfkM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCATAACCTCCCGC, downstream forward: _UP4_TAAGAAAAAACGGACGCTCT

References

• 10220166,24330391,12354229,15805528,22582280