SubtiBank SubtiBank
yfkM
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yfkM

glyoxalase III-like enzyme, general stress protein, survival of salt, paraquat and ethanol stresses
Locus
BSU_07850
Isoelectric point
4.72
Molecular weight
18.72 kDa
Protein length
172 aa Sequence Blast
Gene length
519 bp Sequence Blast
Function
detoxification of methylglyoxal
Product
glyoxalase III-like enzyme
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

    • Gene

    Coordinates
    859,745 → 860,263

    The protein

    Catalyzed reaction/ biological activity

  • methylglyoxal -→ D-lactate PubMed

    • Protein family

  • peptidase C56 family (according to UniProt)

    • Paralogous protein(s)

  • YraA

    • Domains

  • PfpI endopeptidase domain (aa 3-171) (according to UniProt)

    • Structure

  • 1OI4 (from E. coli, 64% identity)

    • Expression and Regulation

    Operons

    Genes
    yfkM
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

    • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-C264 (yfkM::erm), available at the NBRP B. subtilis, Japan
  • BKE07850 (ΔyfkM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCATAACCTCCCGC, downstream forward: _UP4_TAAGAAAAAACGGACGCTCT
  • BKK07850 (ΔyfkM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCATAACCTCCCGC, downstream forward: _UP4_TAAGAAAAAACGGACGCTCT

    • References

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