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spsL [2019-06-27 16:31:22]
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spsL [2019-06-27 16:31:22]

dTDP -4-dehydrorhamnose-3,5-epimerase, spore coat polysaccharide synthesis
locus
BSU37810
pI
4.88
mw
17.54 kDa
protein length
151 aa Sequence Blast
gene length
456 bp Sequence Blast
function
rhamnose biosynthesis, spore coat polysaccharide synthesis
product
dTDP -4-dehydrorhamnose-3,5-epimerase
essential
no
ec
5.1.3.13
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.7|Biosynthesis of rhamnose (for the exosporium)]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    3,882,979 → 3,883,434

    The protein

    Structure

  • [PDB|3RYK] (dTDP-4-dehydrorhamnose 3,5-epimerase (''rfbC'') from ''Bacillus anthracis'' str. Ames with TDP and PPi bound, 32% identity, 56% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • BKE37810 (Δ[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAATTTTTTCACCTTGA, downstream forward: _UP4_TAAAAATAGGAACGTGATCA
  • BKK37810 (Δ[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAATTTTTTCACCTTGA, downstream forward: _UP4_TAAAAATAGGAACGTGATCA

    • References

  • 9353933,15383836,22960854,25239894,26577401