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spsL
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spsL

dTDP -4-dehydrorhamnose-3,5-epimerase
Locus
BSU_37810
Isoelectric point
4.88
Molecular weight
17.54 kDa
Protein length
151 aa Sequence Blast
Gene length
456 bp Sequence Blast
Function
rhamnose biosynthesis, spore crust polysaccharide synthesis
Product
dTDP -4-dehydrorhamnose-3,5-epimerase
Essential
no
E.C.
5.1.3.13
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of rhamnose (for the exosporium)
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    3,882,979 → 3,883,434

    Phenotypes of a mutant

  • production of spores lacking a visible crust PubMed

    • The protein

    Structure

  • 3RYK (dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Bacillus anthracis str. Ames with TDP and PPi bound, 32% identity, 56% similarity)

    • Expression and Regulation

    Operons

    Genes
    spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigK: sigma factor, PubMed, in SigK regulon

    • Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon

    • Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • BKE37810 (ΔspsL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAATTTTTTCACCTTGA, downstream forward: _UP4_TAAAAATAGGAACGTGATCA
  • BKK37810 (ΔspsL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAATTTTTTCACCTTGA, downstream forward: _UP4_TAAAAATAGGAACGTGATCA

    • References

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