resA

extracytoplasmic thioredoxin, cytochrome c biogenesis, reduces disulfide bonds in apo-cytochrome prior to the attachment of heme

locus: BSU_23150

pI: 8.37

mw: 20.76 kDa

protein length: 179 aa

gene length: 540 bp

function: cytochrome c biogenesis

product: extracytoplasmic thioredoxin

essential: no

synonyms: ypxA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.4|Respiration/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,420,804 → 2,421,343

Phenotypes of a mutant

• essential according to [http://www.ncbi.nlm.nih.gov/pubmed/12682299 Kobayashi et al.], but inactivation of ''resA'' was reported by [http://www.ncbi.nlm.nih.gov/pubmed/12637552 Erlendsson et al.] and by [http://www.ncbi.nlm.nih.gov/pubmed/26735940 Meeske et al. ]
• inactivation of ''[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]'' reduces sporulation efficiency to 45.6% that of wild type cells; delayed entry into sporulation [Pubmed|26735940]

The protein

Protein family

• [SW|Thioredoxin family] (according to UniProt)

Paralogous protein(s)

• [protein|0B299F9459023306FA91298A6162A09E4A87C3B2|StoA], [protein|50BBC8AB77B99B9FE5B8A8FE906D39DFEA4ED652|YneN]

[SW|Domains]

• [SW|Thioredoxin domain] (aa 36-174) (according to UniProt)

Structure

• [PDB|3C73]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|22383849]

• genes: [gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|8631715]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8631715], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [PubMed|8631715,11222591], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9988472], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16825793], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]

Biological materials

Mutant

• BKE23150 (Δ[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCCACTGCCCCCTTC, downstream forward: _UP4_ATAAAACCCGGAGAGACTTC
• BKK23150 (Δ[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCCACTGCCCCCTTC, downstream forward: _UP4_ATAAAACCCGGAGAGACTTC

References

• 8631715,18422485,18456809,8631715,11222591,9988472,16825793,16971393,12637552,26735940