ydhU/1

similar to Mn catalase, inactive pseudogene in strain 168

locus: BSU_05890

pI: 0.00

mw: 0.00 kDa

protein length: 242 aa

gene length: 729 bp

function: unknown

product: unknown

essential: no

synonyms: ydhU

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.9|Resistance against oxidative and electrophile stress/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.10|Pseudogenes]

Gene

Coordinates: 633,923 → 634,651

Phenotypes of a mutant

• thsi is a pseudogene in ''B. subtilis'' 168

The protein

Protein family

• manganese catalase family (according to Swiss-Prot)

Structure

• [PDB|2CWL] (pseudokatalase from Thermus thermophilus, 44% identity)

Expression and Regulation

Operons

• genes: [gene|BDDD52CE09533CFD6557B933F2AE1C9B94F9708B|ydhU/2]-[gene|F7C5C38298E1F1EF13A11332C92EA624D9381A3E|ydhU/1]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

Biological materials

Mutant

• MGNA-C196 (ydhU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2194 NBRP B. subtilis, Japan]
• BKE05890 (Δ[gene|F7C5C38298E1F1EF13A11332C92EA624D9381A3E|ydhU/1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATTAGAGCTTTGGA, downstream forward: _UP4_TAATCCAGTGGGAAGGTGCT
• BKK05890 (Δ[gene|F7C5C38298E1F1EF13A11332C92EA624D9381A3E|ydhU/1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGAAATTAGAGCTTTGGA, downstream forward: _UP4_TAATCCAGTGGGAAGGTGCT

References

• 12354229