lysC [2019-07-10 17:06:33]

aspartokinase II (alpha and beta subunits)

Locus: BSU28470

Isoelectric point: 4.64

Molecular weight: 43.65 kDa

Protein length: 408 aa

Gene length: 1227 bp

Function: biosynthesis of lysine

Product: aspartokinase II (alpha and beta subunits)

Essential: no

E.C.: 2.7.2.4

Synonyms: ask,aecA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine

Gene

Coordinates: 2,909,520 → 2,910,746

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate --> ADP + 4-phospho-L-aspartate (according to UniProt)

Protein family

• aspartokinase family (with DapG and ThrD, according to UniProt)

Paralogous protein(s)

• DapG

Domains

• two C-terminal ACT domains (aa 264 ... 337, and aa 343 ...408) (according to the Interpro database)

Structure

• 2RE1 (from Neisseria meningitidis mc58, 40% identity, 58% similarity)

Localization

• cytoplasm (according to UniProt)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: lysC
  Description: PubMed

  Regulatory mechanism

  • L-box: transcription termination/antitermination, via a riboswitch, in L-box

  Regulation

  • expression activated by glucose (5.4 fold) PubMed

Biological materials

Mutant

• BKE28470 (ΔlysC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG
• BKK28470 (ΔlysC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG

References

Reviews

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The L-box riboswitch

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