lysC

aspartokinase II (alpha and beta subunits)

locus: BSU_28470

pI: 4.64

mw: 43.65 kDa

protein length: 408 aa

gene length: 1227 bp

function: biosynthesis of lysine

product: aspartokinase II (alpha and beta subunits)

essential: no

ec: 2.7.2.4

synonyms: ask,aecA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]

Gene

Coordinates: 2,909,520 → 2,910,746

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)

Protein family

• aspartokinase family (with [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|DapG] and [protein|548E92526BC00999031D6EDE43FE061CE0448AFC|ThrD], according to UniProt)

Paralogous protein(s)

• [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|DapG]

[SW|Domains]

• two C-terminal [SW|ACT domain]s (aa 264-337, and aa 343-408) (according to UniProt)

Effectors of protein activity

• feedback inhibition by lysine [pubmed|15033471]

Structure

• [PDB|2RE1] (from ''Neisseria meningitidis mc58'', 40% identity, 58% similarity)

[SW|Localization]

• cytoplasm (according to UniProt)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983], also degraded upon ammonium or amino acid starvation [Pubmed|2168395]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]
  description: [Pubmed|19636616]

  regulatory mechanism

  • [regulon|L-box|L-box]: transcription termination/antitermination, via a riboswitch, in [regulon|L-box|L-box]

  regulation

  • expression activated by glucose (5.4 fold) [Pubmed|12850135]

Biological materials

Mutant

• BKE28470 (Δ[gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG
• BKK28470 (Δ[gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG

References

Reviews

• 19946135,22079167

Research papers

• 15033471

The [SW|L-box] [SW|riboswitch]

• 14523230,14597663,19636616,21169337,22416067,23067368,31045204,31267846,15033471