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dnaG [Tue Sep 06 2016 17:33:08 GMT+0200 (CEST)]
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dnaG [Tue Sep 06 2016 17:33:08 GMT+0200 (CEST)]

DNA primase, part of the replisome
locus
BSU25210
pI
6.00
mw
68.00 kDa
function
DNA replication
product
DNA primase
essential
yes
ec
2.7.7.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|essential genes]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigH regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,601,528 -> 2,603,339

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • required for bacteriophage SPP1 replication [Pubmed|23268446]

    • Protein family

  • DNA primase family (according to Swiss-Prot)

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 262-343) [Pubmed|9722641]

    • Effectors of protein activity

  • the enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' [Pubmed|17350574]
  • [protein|search|DnaG] primase activity is stimulates by [protein|search|DnaC] [Pubmed|23563155]

    • Structure

  • [PDB|1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
  • [PDB|1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
  • [PDB|4M4W] (the [protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaG] complex) [Pubmed|24048025]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • [SW|Interactions]

  • part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
  • part of the [SW|primosome]: [protein|search|DnaA]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|DnaB] [Pubmed|22797751]
  • [protein|search|DnaE]-[protein|search|DnaG] [Pubmed|23563155]
  • [protein|search|DnaG]-[protein|search|DnaC] [Pubmed|23563155]

    • Expression and Regulation

    Operon

  • ''[protein|search|yqxD]-[protein|search|dnaG]-[protein|search|sigA]'' [Pubmed|3298998]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|3298998], [protein|search|SigH] [Pubmed|3127379]

    • Regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

    • Regulatory mechanism

  • [SW|Spo0A]: transcription repression [Pubmed|14651647]

    • Additional information

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' [PubMed|17350574]

    • References

    Reviews

  • 22797751,17350566,27149325

    • Original publications

  • 3919021,20122408,14651647,16479537,19415803,17350574,23563155,23268446,21419346,24048025,9849877,9722641