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dnaG [2017-06-05 16:00:37]
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dnaG [2017-06-05 16:00:37]

DNA primase, part of the replisome
locus
BSU25210
pI
6.71
mw
68.62 kDa
protein length
603 aa Sequence Blast
gene length
1809 bp Sequence Blast
function
DNA replication
product
DNA primase
essential
yes
ec
2.7.7.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,601,528 → 2,603,339

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • required for bacteriophage SPP1 replication [Pubmed|23268446]

    • Protein family

  • DNA primase family (according to Swiss-Prot)

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 262-343) [Pubmed|9722641]

    • Effectors of protein activity

  • the enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' [Pubmed|17350574]
  • [protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] primase activity is stimulates by [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC] [Pubmed|23563155]

    • Structure

  • [PDB|1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
  • [PDB|1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
  • [PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|DnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] complex) [Pubmed|24048025]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
    description
    [Pubmed|3298998]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

    • additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]
    • view in new tab

    References

    Reviews

  • 22797751,17350566,27149325,28075389

    • Original publications

  • 3919021,20122408,14651647,16479537,19415803,17350574,23563155,23268446,21419346,24048025,9849877,9722641,28386108