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pyk [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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pyk [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

pyruvate kinase, glycolytic enzyme
locus
BSU29180
pI
4.00
mw
61.00 kDa
function
catabolic enzyme in glycolysis
product
pyruvate kinase
essential
no
ec
2.7.1.40
synonyms
pykA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,984,788 -> 2,986,545

    Phenotypes of a mutant

  • unable to grow with non-[SW|PTS] carbohydrates (such as glucitol or glycerol) as single carbon source
  • suppression of ''[protein|search|ftsZ]''(ts) mutation (reverted by addition of pyruvate) [Pubmed|24825009]

    • The protein

    Catalyzed reaction/ biological activity

  • ADP phosphoenolpyruvate --> ATP pyruvate
  • The reaction is irreversible under physiological conditions

    • Protein family

  • PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)

    • Modification

  • phosphorylation on Ser36 [Pubmed|17218307], [Pubmed|16493705], phosphorylation on Ser536 or Ser546 [Pubmed|17726680], please note that the Ser is not on position 536 but rather at 538

    • [SW|Cofactors]

  • Mg2, K

    • Effectors of protein activity

  • Activated by PEP (Hill Coefficient 1,8) [Pubmed|4623707] [Pubmed|3711058]
  • Allosterically activated by AMP [Pubmed|3711058]
  • Activation by r5p and ADP [Pubmed|3711058]
  • Inhibition by ADP and f16bp in high concentrations; and ATP [Pubmed|3711058]

    • Structure

  • [PDB|2E28] (Geobacillus stearothermophilus)

    • [SW|Localization]

  • cytoplasm [Pubmed|16479537]

    • Additional information

  • The enzyme is a tetramer with four active sites [Pubmed|3711058]

    • Expression and Regulation

    Operon

  • ''[protein|search|pfkA]-[protein|search|pyk]-[protein|search|ytzA]'' [Pubmed|11489127]

    • Regulation

  • twofold induced by glucose [Pubmed|11489127]

    • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP589 (''pyk''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP600 (''pyk''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP1745: ''pyk::aphA3'', available in [SW|Jörg Stülke]' lab

    • Expression vector

  • expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', native protein: pGP1411 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see ''[protein|search|pfkA]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

  • 17726680,16493705,11489127,17505547,10966427,17218307,3711058,4623707,21821766,22846916,23420519,24158146,24571712,15378759,24825009