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birA [Tue Mar 01 2016 10:08:20 GMT+0100 (CET)]
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birA [Tue Mar 01 2016 10:08:20 GMT+0100 (CET)]

trigger enzyme: transcriptional repressor (BirA family) of the biotin operon / biotin-protein ligase
locus
BSU22440
pI
6.00
mw
36.00 kDa
function
regulation of biotin synthesis, addition of biotin to proteins
product
trigger enzyme: transcriptional repressor (BirA family)/ biotin-protein ligase
essential
yes
ec
6.3.4.15
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.3|Trigger enzymes that act directly as transcription factors by binding DNA]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids], [SW|biosynthesis of cofactors], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|essential genes]

    • This gene is a member of the following [SW|regulons]

  • [SW|Spx regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,354,918 -> 2,355,895

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • transcriptional repressor of the ''[protein|search|bioW]-[protein|search|bioA]-[protein|search|bioF]-[protein|search|bioD]-[protein|search|bioB]-[protein|search|bioI]-[protein|search|ytbQ]'' operon [Pubmed|8763940,8892842] '''and
  • ATP biotin apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP diphosphate [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] (according to Swiss-Prot)

    • Protein family

  • ([SW|BirA family]), pyrroline-5-carboxylate reductase family (according to Swiss-Prot)

    • Structure

  • [PDB|4HA8]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [SW|BirA]-[SW|AccB] [Pubmed|24816803]
  • [SW|BirA]-[SW|PycA] [Pubmed|24816803]
  • [SW|BirA]-[SW|YngHB] [Pubmed|24816803]

    • Expression and Regulation

    Operon

  • ''[protein|search|ypjD]-[protein|search|dapB]-[protein|search|mgsA]-[SW|bshB1]-[protein|search|bshA]-[protein|search|cca]-[protein|search|birA]'' [Pubmed|20308541]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|23894131]

    • Regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

    • Regulatory mechanism

  • [protein|search|Spx]: transcription activation [Pubmed|23894131]

    • Biological materials

    Expression vector

  • pGP2690: expression in ''E. coli'', with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]' lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 12368242,21437340,26350309,18086213

    • Original publications

  • 7730294,8763940,8892842,11717296,20308541,23894131,24189073,24816803,22437830