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birA

trigger enzyme

locus: BSU_22440

pI: 6.77

mw: 36.03 kDa

protein length: 325 aa

gene length: 978 bp

function: regulation of biotin synthesis, addition of biotin to proteins

product: trigger enzyme

essential: yes

ec: 6.3.4.15

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.3|Trigger enzymes that act directly as transcription factors by binding DNA]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,354,918 → 2,355,895

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• transcriptional repressor of the ''[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]'' operon [Pubmed|8763940,8892842] '''
• ATP + biotin + L-lysyl-[protein] --> AMP + diphosphate + H⁺ + N⁶-biotinyl-L-lysyl-[protein] (according to UniProt)

Protein family

• biotin--protein ligase family (single member, according to UniProt)

[SW|Domains]

• [SW|BPL/LPL catalytic domain] (aa 74-262) (according to UniProt)

Effectors of protein activity

• biotinoyl-5'-AMP acts as co-repressor for DNA binding [pubmed|28466579]

Structure

• [PDB|4HA8]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
  description: [Pubmed|20308541]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Biological materials

Expression vectors

• pGP2690: expression in ''E. coli'', with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]' lab
• pGP2700: expression in ''B. subtilis'', with N-terminal Strep-tag, in [SW|pGP380], available in [SW|Jörg Stülke]' lab

lacZ fusion

• pBP629 (in [SW|pAC7]), available in [SW|Fabian Commichau]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Jörg Stülke]'s lab

References

Reviews

• 12368242,21437340,26350309,18086213

Original publications

• 7730294,8763940,8892842,11717296,20308541,23894131,24189073,24816803,22437830,28466579